FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677353
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA8959.253563E-811.3400461
CTCCAAT6154.0297833E-410.6090671
CCCGCTT5550.002048242410.4497721
CATAAAC7803.425055E-49.2942691
CTTAAAT6650.0091064248.7212371
CTTAAAA10055.0716808E-58.6561551
CTCCAGC9302.0801567E-48.5747121
CTTTCAG11753.5750832E-58.0207561
CTTTATT14909.0181245E-56.8116381
CTTTTAT15801.9369494E-46.4236331
CTTTTCG12550.0050358136.3541691
CTTTTAA13400.00993453155.9511061
Produced by FastQC (version 0.11.5)