FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742791
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAG2100.00991994217.4279421
CTCAAAG7853.316231E-49.3245041
CTTTCTA8608.2435145E-48.5113211
CATACAT8050.00430825078.1835551
CTCCTTT15001.1015329E-57.31973551
CTTCTAT11200.00137594447.18902541
CTTTGAT15401.5948532E-57.12961241
CTCAAAA10600.00641624686.9054111
ATATTGC13752.6123517E-46.8300866
TCTTATA10900.0095273716.62853432
CCCCCCC3459.07462E-56.294056125-129
CTTTATT18700.00148296865.48001581
CTTTCTT23451.5655706E-45.3064181
Produced by FastQC (version 0.11.5)