FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805108
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGT300.001940066272.457911
CCCCTAT2851.6397728E-417.796681
CTTCAAT12950.012.8689731
CCCGCTT6403.4078184E-612.4537041
CTCAAAT6455.045306E-511.2337861
CTTCCGA9502.1263986E-710.6780081
CCTCAAT6850.00109106659.5200181
CTCCAAT6650.0091480548.7167411
CAACAGA7850.0037638358.3072771
CCTCCAT9350.00208263597.7495091
CTTCATT12305.303563E-47.06906461
CTTCAAG10250.00511322457.06906461
CCTCTTT10750.008110536.74027061
GATTTCC16354.4802095E-56.62150435
CTTTATT12900.00674306146.1785821
CTTCTTT22652.7296392E-66.07814741
CCTGCTT14600.003678935.95544531
CTTTTTT27252.9425428E-75.84981351
CTTTCCG16800.00282183245.60686251
CTTTTCT18200.00724161045.1755651