FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174307
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3800.039.8998767
CACGTCT4950.032.088799
ACACGTC5000.031.7679028
AGAGCAC8100.021.395263
GCACACG7800.019.4384026
GAGCACA8600.018.472074
AGCACAC9750.016.2912335
AAGAGCA17700.010.2024872
GAAGAGC16551.8189894E-1210.0792981
GTCCCAT6400.00663374889.065891
CTCGGCC10508.43802E-58.2888141
CACCGTT12204.783505E-47.1338151
AGTCACA5801.0913936E-116.99006120-24
GTCTCAC5203.5470293E-106.95858230-34
CTTTTAT18202.6855012E-56.376011
AAATTGA15500.00112533516.05623874
CTGAAAT18602.4713945E-45.8489611
AGTCTCA5852.0117477E-75.687855225-29
ACTCCAG6251.3464705E-75.54517915-19
ACGTCTG7558.1599865E-95.355261310-14
Produced by FastQC (version 0.11.5)