FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2095699
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT12458.8311936E-99.8757611
GATTAGT6450.0073893498.9481227
CTCTGGG9600.00275145037.53389931
CTCATGA10900.00117400067.29889631
CCCTTAT12004.090793E-47.23254251
CTCGTCT10150.00473942847.1256581
CTTTGAT29101.2732926E-116.95914751
CTTATAG13953.0819076E-46.7400041
GTTCAAC18605.4534576E-66.61038881
GTCACAT12450.0047591256.3901981
CTCAAAT15852.0883475E-46.38836571
CAACCGT12550.0051729256.33928061
CCTCGAT19451.09152115E-56.32150271
CCGTAAT15500.00110510036.06600331
CTGTAAT19105.6664903E-56.05867431
CGTTTAC14350.00319125746.0329685
CTTCGAT20402.276366E-56.02711961
GATCCAT13200.0089645196.012025
CTTTAAT26551.1685133E-65.7206551
CAACTAT16500.00233391745.6983671
Produced by FastQC (version 0.11.5)