FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489197
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTGTATCTCGTATGCCGT6850.14002538854490112TruSeq Adapter, Index 22 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1950.068.394668
CACGTCT2300.057.9867829
GCACACG2850.046.81076
CACACGT2900.045.9895137
AGAGCAC3700.039.8523523
AGCACAC3350.039.8240285
GAGCACA4500.029.6467784
AAGAGCA7250.020.3488452
GAAGAGC7100.019.835961
CCCAGGT2156.1469374E-419.651441
CCAGTCA3450.010.18769820-24
GTTTATC6050.0053156879.3114251
CAGTCAC3054.674803E-109.21903120-24
ACTCCAG3404.1836756E-119.08399715-19
CTCGTAT3551.0186341E-108.70835535-39
TCTCGTA3352.7685019E-98.38932435-39
ACGTCTG3201.1041266E-88.335610-14
CGTCTGA4051.5334081E-97.626071510-14
TCGTATG4101.9299478E-97.53954440-44
TATCTCG3802.2536733E-77.02605935-39
Produced by FastQC (version 0.11.5)