FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930412
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3356.720256E-821.2347039
CACACGT3651.6611011E-719.4893847
ACACGTC3953.8108374E-718.0091788
GCACACG4951.2332748E-817.2451526
GAGCACA5156.036362E-613.8150934
AGAGCAC5156.0430775E-613.8136073
AGCACAC5651.5715541E-512.59048755
AAGTTTA9550.00306622267.4500244
GAAGAGC10700.00111977957.3304091
AAGAGCA12751.242625E-47.25622842
CTTAAAA12850.00765178866.103921
CTTTTAT20904.0848372E-55.7999191
CTTCCAT19000.0025216625.25404451
CTTTTCT23852.9844767E-45.0825291
Produced by FastQC (version 0.11.5)