FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641276
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4800.027.0787667
ACACGTC6750.020.326418
CACGTCT6900.019.8845319
AGCACAC10151.8189894E-1213.516745
GCACACG10301.8189894E-1213.3198956
AGAGCAC10554.5474735E-1112.3198213
GAGCACA10958.731149E-1111.8697824
GAAGAGC20601.1150405E-97.74573331
GTTCAAT14255.0074665E-57.12558031
CACAATT11750.0025200746.78987651
CTCGGAA11800.00263516056.76110651
AGTCACT5252.1866072E-86.34344120-24
GTTTGTT14906.6335895E-46.3279661
CACCGCT13950.00218988726.23898741
CCCAGCT16302.8917755E-46.22941881
AAGAGCA23307.0914757E-76.20168832
ATTGTAG14450.00341356645.99649956
CCCAAAA15850.00139637295.9486871
CTTTCAA22851.2223318E-45.3959731
CTTTCAT32301.3750196E-65.16454841
Produced by FastQC (version 0.11.5)