FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032677
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGATT12156.3581247E-46.95910261
CTTCGAT20801.4352554E-76.7750881
ATTGATC11900.00396568936.50277662
GTTCGAT15752.6978913E-46.2631921
CCCAATT12650.0073682376.1270361
GTTGGAT15050.0010614936.08632471
CGAAGAT22804.9355203E-65.87174221
CTCTGAA14650.0052254295.77154251
GTTCCAT29604.3126965E-75.47500371
CCACCGT17300.0055597065.29475071
CAGAACC21805.6030345E-45.16291863
CACCAAC20750.00158750535.09355971
AATAAGC19550.0042740825.03735265