FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699865
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCT8250.0061193217.87148144
GTTTAAA15652.8799823E-67.41352941
CGAAGGT9800.00328119847.3993451
CTTGAAT17701.2593283E-46.1452191
CAAAGGG13300.0091908935.9973641
CTTGGAA22451.5161282E-55.81400631
CCTGGAA15300.00614764735.687341
CTTAAAA22355.185952E-45.19112871
GGCAAAT22355.185952E-45.19112871