FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515395
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2450.006214149828.853373145
CAATAGG3656.323353E-810.478801135-139
CCAATAG3403.2189448E-510.3956995145
ACCCAAT3555.0341943E-49.956445145
AATAGGA3952.0842272E-69.037413135-139
AATTCCG5750.0069662749.0103055
AATATTC7000.00368161438.3264885
TCAGGAA7200.0047457958.0951978
CACCCAA4454.2089614E-67.8364153130-134
CGATGAT8350.00195143287.79976751
AGGATCT5452.6602994E-46.8208714140-144
GATCTCG6101.4694296E-46.5628304140-144
GGATCTC4800.00365540156.553064140-144
CCCAATA5150.00760675556.1077104140-144
TCACCCA5952.405643E-45.8608484130-134
Produced by FastQC (version 0.11.5)