FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239291
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGT5250.001398367610.9409451
CTCAATT6000.00423682439.5733261
CGAATGA6600.0092725418.7030251
GAAACTC7750.00378681428.3023994
CTGCACT12055.534778E-57.74605271
CACCGTT14055.7528396E-67.66547541
CTTGAAT9950.00418906337.2160751
TTGAATT15352.1062795E-56.9907882
CTACAAG10350.00614406876.93719341
CCGATCT10350.0064021446.9078039
CGTTCTC10450.00702944356.84142354
CTCGGCA12859.700511E-46.70505331
CCCTGAT11900.0031996066.63697051
CTTAAAA14150.00287494656.08904171
TTACCGG17751.5873145E-46.04189548
AATTTTG19207.218981E-55.957745
CAAGAAC16150.00196992075.7795631
CTTTCTT30207.354356E-85.70595651
CTTTGAA20501.7444069E-45.6038991
ATTTTGT23453.8151935E-55.48772576
Produced by FastQC (version 0.11.5)