FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125954
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT9501.03682396E-1012.9074887
CACGTCT12509.8680175E-99.80968959
ACACGTC14501.098997E-78.4566298
AGCACAC18501.2551027E-98.1876075
GAGCACA20357.998096E-97.4438754
GCACACG20607.862036E-87.00280766
AGAGCAC22302.2727909E-66.14600473
TAAGGTT13050.0079845676.07992949
TAGTAAT14300.00307726636.0528739
GTTCGAA24952.371511E-65.79662041
CTCCTAT15200.00589980845.70890761
CAATTTA15800.0092922335.47858434
GTTACGA17550.00485418835.35650631
CCCCGAT23058.332475E-45.0195461
Produced by FastQC (version 0.11.5)