FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678585
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC3800.001493595213.3052339
ATAGCTA4050.002411757412.4839238
CCTCGAA5059.3365676E-411.4810431
CTCCGCT5150.001100211611.2581091
CAACCTT5852.5438386E-411.1498581
CTTCGAC6103.7472174E-410.6928981
CTTCGAT7000.00132663279.3180961
CTTCGGG7150.00160964119.1226121
CTCCAGA6600.0085814648.7847381
CTCATTT7600.00280225678.5824581
CTTTGAT11352.3253155E-58.3009961
GTTTTAT11502.7414972E-58.1927221
AGATTGA9753.596726E-48.1506484
CTTCTCT8100.0049801378.0526761
GGAAAGG9050.00150651818.0081861
CTTTATA13001.2596596E-47.2474081
CTTTTAT16152.5824903E-46.28258371
CTATTTT14100.00248386946.1680071
Produced by FastQC (version 0.11.5)