FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500266
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2054.9698247E-727.6242859
ACACGTC1805.6529916E-627.528378
CACACGT2051.5507716E-524.171257
GAGCACA3452.1830147E-618.4680824
GCACACG3555.75163E-515.9536496
AGAGCAC3255.3228106E-415.2480063
CTCCGAA3305.815525E-415.0712161
AGCACAC3509.334069E-414.1588635
AAGAGCA7150.00198705968.9129542
ATCTGCC8851.5695662E-48.79929356
ATTTTTA14800.00552895575.7400795
Produced by FastQC (version 0.11.5)