FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500666
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC6400.028.9431348
CACGTCT6300.028.271689
CACACGT7050.026.2746167
AGAGCAC10900.020.2615623
AGCACAC10600.019.4908075
GCACACG9950.019.3320716
GAGCACA11950.017.290074
GAAGAGC19700.010.8758931
AAGAGCA24000.09.5015012
CTCGAAA9000.0016513877.9353741
CTTGCAT12206.9006026E-57.6101531
CCACGAT14658.818372E-56.8249631
CACAAAT14150.00305272946.05668071
AGTCACT6856.51562E-95.616028320-24
ACGTCTG8201.5161277E-85.038952410-14
Produced by FastQC (version 0.11.5)