FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524785
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2256.973441E-419.2869591
CTCAAAA6705.790469E-611.87443351
CTCCAAG4350.00407451311.6386811
CCTTGCT5200.001212535411.127091
CCAGAAT6400.0067761569.0407621
ATTGATG10400.00636762666.91003476
CTTTCAT10600.0071964686.8232161
CAATAAT10800.0086204936.696861
AGTAGTA2600.0079711716.080830610-14
Produced by FastQC (version 0.11.5)