FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734799
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT16901.4525976E-67.192541
GTTAAAG20757.6722245E-76.54719921
GGTGAAG40100.06.240851
GTTCGCT14300.0033924756.0001941
GGTGAAT22904.1483036E-65.9325061
CCCGGTT24109.832718E-65.6371111
GGTTGAT24201.0541573E-55.61381771
GTTACAT19204.6185704E-45.5861181
GGTAAAG35601.46828825E-85.4229281
CTCAAGA18550.00180685595.3964011
CTTGGTT17250.0045242215.388581
GTTCGAT18700.00199959945.3531141
CTCCAAT24758.966961E-55.2001681
CTTGAAT28957.0561873E-65.18669561
GGTAAAC23452.2342926E-45.1835361
CTTTAAG22406.5303256E-45.1073081
CACCCAT19650.0037177955.09431271
GTGAAGT22557.418888E-45.0611792
GGTGATT26906.122203E-55.0503491
Produced by FastQC (version 0.11.5)