FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714351
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAG5009.0301945E-411.5270081
CCCCATT4750.0080797110.61698151
CTTTAGA7550.00278628388.5880021
CAATCTT9954.762546E-47.94065484
CTATAAA10901.4068699E-47.93142751
CTTATAA10609.072606E-47.47624351
CTTTAAT14909.789269E-56.7692161
CTTTGAA12300.00435814176.44294171
CTTTTAC12450.0049455936.3653161
CTTCAAA14000.0024522036.1751831
CTTTTCT18804.7424455E-56.13138771
CTTTTAT20101.915198E-56.09325651
ATTTTAC14950.00527467165.7653645
ATTAAAT20900.00129577965.1706081
ATTGTTT19650.00350109985.1174333
Produced by FastQC (version 0.11.5)