FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980124
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA6658.4121386E-49.7946341
CTCCAAT9803.7010286E-69.6002971
TTACCCG6450.00742221538.9424199
GTTAAAA10951.4091373E-47.93111941
CTCAAAA12951.4475758E-57.8239421
CTTTGAA17151.5077294E-67.17384861
GTCAGGA10300.00542574477.02635571
ATCGGAT10950.0098047226.6092661
CTTATAA15650.00122899736.01168781
AGATATA16250.00201306665.76874354
CTTTGAT19103.6450897E-45.68362331
ATCAAAT17859.5079123E-45.67619371
CAATATA17450.00468140755.3720394
TTCAGAT17950.0064908935.2245322
ATTAAAT18200.00734833675.16939071
CTTTCCG18250.0075876015.15522771
CTTTTAT26051.6495763E-45.00071531