FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816247
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC6101.8189894E-1218.9100559
AGTGAGT5107.2941475E-1018.4389861
GTGAGTA5955.84987E-915.7643452
ATAACCC11352.537272E-79.5279198
CTACTGG8600.0086781517.57020851
GAGTATA16851.2135079E-67.2744394
CTCCCAG10550.0068835646.85664031
TATAACC20052.6568068E-66.472517
TGAGTAT16957.502162E-56.3809313
CTTTCGT17108.103106E-56.34539941
CTTCCGA17601.2048233E-46.16513251
CAACACT13250.0090657856.0053821
ATACAAG19003.577598E-45.6923056
CCCGGCT15250.0060912295.69213531
CTTATCG15300.00631582975.6735341
AACCCCC4602.4623221E-55.641874310-14
GTATAAC19254.2697124E-45.6183786
CTTGAAT22751.1908691E-45.40544371
CTTTAAC24305.5806333E-55.35833741
CTCCAAT18000.0065024935.22437861
Produced by FastQC (version 0.11.5)