FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423105
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC9702.6451744E-811.11912258
CTTCCGA12257.95053E-78.804226
CCGATCT14501.0367748E-67.9341979
CTTTAAT11101.6795208E-47.81262251
GGTACTG9700.00322290657.41274837
ACGCTCT13201.5793268E-47.11718131
CTCTTCC19351.5483474E-66.68847754
TTCCGAT16153.9878614E-56.67835867
CAGAATT12500.0052729966.3272994
GATAACG12900.0073114086.13131957
CGCTCTT16804.730964E-45.9955532
CTCACCG18300.0013295375.5286041
GCTCTTC19706.0504896E-45.47470573
CTTTGAC18500.00152534065.4688361
CTTTTAT20258.17924E-45.3530931
GAACTGC21753.9767206E-45.28927855
Produced by FastQC (version 0.11.5)