FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842230
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTGT4452.9713113E-413.1545051
CTTGGAT4906.692571E-411.9464371
CCCGATT5701.8295574E-411.5534621
TAGACGA4650.006749205810.8777584
CGCACAT5750.002545493710.1804421
CCCAACG6250.0050722019.3660071
ATTCGCT6450.00657241149.0755881
CCTTGAT12154.8397094E-57.8290951
CTTTAAA10355.959007E-47.77672671
CTTGAAT10150.0042254817.2090571
CTCAAAA10250.0046486617.13872431
CTTTTAA11000.0092112416.65199331
TTAAAGA10900.0095252916.62890862
CAAAAAA22152.2533641E-76.6069461
CTTCCAT13750.00168241796.3859141
AAGGATT13000.0075168876.1142574
CAAAGAA19305.5474316E-56.06606671
CTTCCTT17054.5955984E-46.00825261
CATCAAA14650.00342633065.9936051
CTTCTTT23202.2142283E-55.67713261
Produced by FastQC (version 0.11.5)