FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223312
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT4001.2547373E-414.562691
CTCAGGA7656.4028427E-1014.2771451
CTCAGCT5109.7603735E-411.4217171
TAGGTCT4650.006824592610.8621529
CCCAAAT11402.3383473E-79.5807161
CATAAGT10004.4331227E-69.4657481
CCCTTAT9251.8172364E-59.4460691
CTTCAAT17805.0931703E-118.9994141
CTCAAAC8951.3105544E-48.9491391
CTTTAAT19002.1827873E-118.814261
CTCATAT12407.895869E-78.8080781
CTCAAAT11001.4822725E-58.6052261
CTCAATT9502.5016163E-48.431031
CTCACCG8900.00122137818.1812861
CTTCGAT12558.780629E-68.1226151
CTCTTGT8500.00736805947.70965861
CTCCAAG9600.00257408927.5847341
CCCATAT12708.9288E-57.45334531
TGATCCA12801.0985654E-47.32951454
CTTTACA13953.5876896E-57.30744271
Produced by FastQC (version 0.11.5)