FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314914
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT21303.6379788E-128.5436521
CTCAAAA54500.07.6130991
CTCAAAT35450.07.18678951
CTCAGCT17952.8322247E-66.89394141
CTCACGT11750.00242792336.8145621
CTCAAGA28208.449024E-85.93692871
CTCTAAA17356.1652093E-45.8736991
CCCAGAT34759.0221874E-105.86524771
CCCAGAA34809.349606E-105.85682061
CCCAAAT31151.735134E-85.8420471
CCCACGT15100.00509785355.7847881
GTTAAAT29051.5417208E-75.7632151
CTTTAGG17808.5475564E-45.7252071
CTTTGAT67850.05.47146271
CCCATGT25351.702496E-55.4558041
CTCAATT27008.487903E-65.39199351
CTCACAA19050.0020174385.3495371
CTCCCAA31457.5680146E-75.32341531
CTTTAAT54700.05.3229921
CTCTTGG17950.00585455965.27183771
Produced by FastQC (version 0.11.5)