FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182929
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA15601.9444717E-57.0320871
CTCACAT16807.315626E-66.96511361
CCTAAAT16253.4389544E-56.7508031
CTCTAAT13059.39519E-46.72493741
CTCGAAT15801.7367803E-46.4802011
CTTAAAG30552.3283064E-106.4635341
CTCAAGA27255.655238E-96.4411331
CTCATGT20051.4719049E-56.2008621
CTTAATT22602.258861E-66.1484081
CTCAGTT19255.4080818E-56.0786451
CTCAAAA50650.06.0643931
CTCTTAT29202.4670953E-86.01098871
CTCGCTT23052.0239477E-55.7110911
CTCTCAT23102.0954787E-55.69872951
CTTCAAT63450.05.6478351
CTTAGCA15650.0071911775.60769561
CTCTAAA17150.00323774765.5436621
CTCTGAT31701.3879071E-75.5369361
CTTTAAT45001.6370905E-115.5256571
CCCTTGA33859.550058E-85.40130661
Produced by FastQC (version 0.11.5)