FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474503
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATTC4705.0651486E-412.34670451
CTCAAAG5301.0055383E-412.3175851
CAACCAA4400.004341074311.539961
CTCCATG4750.0076744810.6896471
CTCAAAA6254.646337E-410.4453121
TGAGTCA1250.005663689.24265255-59
CTTCCAA6350.0062014099.1385061
ACAATTT6400.00682412979.032693
CAAAGGC7500.00254949428.6713833
TCAAACT6700.00993183358.6282423
CTTCTTT14355.4492528E-57.07676941
Produced by FastQC (version 0.11.5)