FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769005
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2755.4379725E-620.9859648
CACGTCT2908.608587E-619.9004829
CACACGT3403.3860604E-516.9739427
AGCACAC4551.4449215E-615.8537495
GCACACG4903.1215022E-614.7222966
GAGCACA4905.060735E-513.2569624
AGAGCAC5158.067394E-512.61342053
GAAGAGC10155.060142E-47.8960881
GTTCTTT14956.522351E-46.3355971
ACTCCAG2900.00359058225.969911615-19
Produced by FastQC (version 0.11.5)