FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509182
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC500.001399743717.389683105-109
CGATATA3559.25322E-414.1752344
AGTACCT3250.00844348213.2717577
GTTAAAG4000.002206840612.6314241
TTAAAGC4857.1155635E-411.8579283
CTTCATT7400.00228113758.7785951
TTTAAAG7700.00338808828.4033992
GATTTTG11100.00151248027.1241045
CAAAGAA13802.753851E-46.7995241
CTTCTTT17701.9039373E-56.52470641