FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980224
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT10558.056721E-58.322481
CCCCCTC8550.0074384477.70194441
CTTTAAC17401.9859908E-77.56915241
CTTCAAT35900.07.5410411
CTAAAAG13702.6510803E-57.477071
CTCACGG10050.00384408867.2804451
CCCAAAT14555.8701087E-57.0402651
CCCATAC13752.2408526E-46.9177461
CCTCGAT13952.6744505E-46.81856731
CTCCTGA17401.2198423E-56.7281351
CTTACTT15501.3436099E-46.60876461
CTACGAT13300.00115800646.60166651
CATCGAG14654.8585387E-46.49276541
CTTAAAT20302.5564023E-66.48784541
GTTTAAT16002.0301541E-46.4022411
CTTAAGA12600.00477799966.3877241
CTTAATT16102.2009233E-46.3624761
CTTTAAA34501.6370905E-116.3624761
CTTCAAG24151.6002741E-76.36247541
CTCCGAT16152.2911247E-46.34277771
Produced by FastQC (version 0.11.5)