FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696213
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT2754.9312002E-621.2237471
CGCTGAG4400.004161332711.6067381
CTTCGAT8003.1434138E-610.9434961
CTCCGAA6455.896486E-410.17999651
GACGATT5750.002844067310.0447852
CTTTAAA8557.779947E-59.3862341
CGAATTT6450.00733499468.9546544
CTTTCAC8255.632227E-48.8432281
AGAATGG6550.0083209068.8179414
CCTCCAT7800.00333817028.4180741
GGAAAAG16553.251467E-46.17155841
CTCTTTT14850.0041156275.8954861
Produced by FastQC (version 0.11.5)