FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817713
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGT6000.0042907789.5591041
ACCGAGC9600.00306984137.44997266
CCCAGAT16559.994146E-77.3642651
CTCATAA19008.698202E-66.4146621
CTCAGAT12600.0059060896.25893741
CCCCGGC13250.0099427845.9518951
CTTCGAA14550.00399301855.91284751
CTCAGGA16000.00179539715.82507941
CTTTTAT38201.2760211E-85.2550051
CAAAAAT46504.1472958E-105.087911