FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212820
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6452.5787267E-713.4613911
CTTCGAT11502.3737812E-910.6959531
CTCAACT5500.001932472510.5243611
CTATTAA7050.00143878039.2368061
CTCAGCT6450.0072109418.9742611
TTCGATC8458.070017E-48.5305812
CCCAGAT12851.4433026E-68.4461061
CTCAAAC9000.00145174688.0394421
CTATAAT11001.4904543E-47.89327051
CTCAAAT11302.0354215E-47.6837151
CTCTTAT9600.002738437.53697731
CTTTAAT17703.1801756E-77.35813331
CTCCATT14054.2855594E-57.20974871
GTTAAAT10300.00543998237.02475551
CAACAGT10400.0059746126.95720961
GTCAAAG14758.1225524E-56.86759141
CTTTGAG12707.7761617E-46.8366911
CACAAAT11900.0029511426.6882761
ATTTAAT20453.4556415E-66.3686541
GTTTGAT13650.00176045456.36087751
Produced by FastQC (version 0.11.5)