FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642173
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCT20751.3096724E-108.0975981
CTCAAAT19703.3342076E-97.78752661
CTCCAAT21702.1565938E-87.06978231
CCCCGCT15752.2557691E-56.95756341
CTCAGAA14857.836982E-56.88728571
CTTAAAG18803.8904098E-56.2173971
CTCCTAT18151.6059175E-46.0375551
CTTTGAT43301.8189894E-125.9050921
CTCCAGT17808.160076E-45.7458531
TCTCGAA18800.0057413935.7227473145
CTTTATT52400.05.716091
CTTAATT15500.0065409555.6558261
CACGAAT15550.0067779155.637641
CTCCCGA18150.0010453025.63505131
GTTCAAT24901.1863343E-55.5744341
CTCCGAT21552.785966E-45.4239941
CTGACTT18950.0018035525.397161
CCCGATT17600.00445064255.39606481
CTACAAG20357.5660914E-45.38484671
CCCGCAT24455.2674368E-55.3782391
Produced by FastQC (version 0.11.5)