FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069788
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3900.031.236317
CACGTCT3900.031.236319
ACACGTC4050.030.0794078
GAGCACA7050.018.2969674
AGCACAC7450.017.313775
AGAGCAC7251.8189894E-1216.8076933
GCACACG7301.8189894E-1216.6878916
AAGAGCA13252.7230271E-99.7376422
GAAGAGC13453.2378011E-99.6406241
AGTCACG2859.1495167E-109.57504420-24
GGACAAT8700.0099785367.4520921
CTAAAAA13200.0090935086.00307371
ACTCCAG5853.9388397E-85.879610515-19
CAGTCAC5209.06497E-75.80026720-24
ACGTCTG5909.557067E-65.101012710-14
Produced by FastQC (version 0.11.5)