FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775548
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAC4503.549074E-412.8805821
CCCCGAA7401.3387416E-611.749181
CCCACAT4400.004382924611.5266571
CTCTCGG4600.0061042311.0254981
CTCCCGC8154.268217E-610.6679671
CTTTGAT11553.0313822E-79.4095161
CTCGTTT6550.0080844188.8492551
GTTTACG6600.0087646688.7623957
TCAATTT10155.8737984E-58.5487773
TATGCAA8558.8185526E-48.4538529
CGGGAAT9100.00159580137.9618981
CTTCAAA12004.6866575E-57.84910441
CTTCAGC12105.1972434E-57.78423641
CGTTCAT8450.0073002837.71691661
CTTTCTC11450.00193663036.96057651
ATTTAAT10550.00676753146.86760851
CTTTTTA14655.459661E-46.42930031
CATCAAC12900.0067468076.1781861
CAAAAAG19507.43102E-55.9448841
CTTGCCG22951.3210591E-45.36690951
Produced by FastQC (version 0.11.5)