FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990065
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT6151.4455327E-714.1169771
CTACAGG5650.002419516710.2441781
CTCTCAT9150.00171121297.9070491
CTAAAAG11850.00282800576.71598771
CTTCCGA19651.8026785E-66.62743471
CTAAAAT17007.457593E-56.38377951
TCCGATC13600.00173243326.3704668
CTACTAT13050.00773404176.09842541
CTATAAT19155.8567137E-56.0448671
CTTCAAT27656.391019E-86.0182231
CTCGAAA15100.00545037445.74962951
CTTCGAT17809.227856E-45.69041061
CTTTAAT21655.6159595E-55.68102361
CTCCAGG15350.00653673665.65598731
CCGATCT18000.00109244295.61530739
TTTAATC15700.0085583255.51947452
CTATGAC18950.00203671475.3450821
CTCTTCC28855.531563E-65.2562834
GAATTAA18000.00663785.21526245
CTTTATT33405.1123607E-75.19876671
Produced by FastQC (version 0.11.5)