FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693902
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAT5402.836532E-816.117331
CGCATGT3100.00582733514.0376761
CCCTTAT4151.7721235E-413.9812981
CCCTCGA4000.002129790112.6923981
CCAGGTT4550.005583274611.1581531
CTCCGAA6600.0085297568.7912711
CCCCGCC6600.0085297568.7912711
GTAAAGA8558.90893E-48.4448872
CAATGAT9100.00157913397.9701091
CCTTTAT8500.00762328557.67943431
CAAGGAT11702.9525888E-47.43876841
CTCTTTT12606.9030427E-46.9074281
CAAAAAG14808.209249E-56.8607561
AAAGAAC10700.00802903256.74704466
CCTTGAT12250.00389377546.51271721
Produced by FastQC (version 0.11.5)