FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752306
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCC3950.002065355412.7446429
CTCAAAT6103.6537176E-410.7224711
CTGAAGG8204.4269746E-610.6352971
GTTTAAT5850.00310680649.9383961
CTCGTCA6100.0043908419.5310861
CTCATTT8406.690428E-59.5169031
TCAGATT11452.8588829E-58.1656992
ATTTTGG9854.2170272E-48.0312496
CCCGTTT8150.00513561448.0254081
CCCAAAG8150.00513561448.0254081
CCTGAAT8200.0054258137.97647241
CTGCTAT8650.0087556097.56151151
CAAAGAT14759.616795E-67.390631
CAACAAG10500.00627449766.92138341
CAAAAAG17902.7629576E-66.902051
CTTTAAT11750.00246275646.80357271
CTTGAAA14103.3016817E-46.7004881
CTTCCAT13100.00105055956.65720841
CTTCCAA12050.00320950786.6341891
CTTTCAT14655.260457E-46.44893361
Produced by FastQC (version 0.11.5)