FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788694
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT7953.2636399E-610.9098161
AAGGACT6500.007953588.8669316
GTCAGAT6550.008247138.827791
ATTTTCG9803.9177356E-48.0865516
CCTTTAT11602.7848093E-47.47698551
CTTCAAA14254.016779E-46.5937391
AGATTTT15401.502905E-46.55068354
CTTTCAA13000.00749587366.1157911
CTTTTCT16754.2836065E-46.04110671
CTCTTTT14550.00364039955.9610331
Produced by FastQC (version 0.11.5)