FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788226
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTGG4000.00216101712.6681941
AATCTAA4856.7952735E-411.9246555
TCGATCC4800.00850857510.543433
CAAACGT4900.009820119510.3413821
CCCACAT5650.002405623410.2498661
CTCTTGA6708.9852937E-49.7239871
CCAAGAT6050.0042380999.5721881
CCTCAAT6200.00518643259.3406041
CTTTGAT12206.779701E-78.9003711
CTCCAAT6700.0098008948.6435431
CTTCCAC8659.749982E-48.3687491
GCTCCTT10050.00484237078.077662145
CTCATAA8100.00503446358.0432981
ATCAGGT9100.00160975247.95490931
CTTGAAG10356.684071E-47.69358871
CTATATT9400.00224277387.69125654
CTATAAA8650.009069167.5318741
CATCAAG13502.506265E-57.50707771
CAACAAG9750.0031692267.42458251
CAAAGAG10850.00110531287.3390451
Produced by FastQC (version 0.11.5)