FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293083
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGGG6550.0084194968.806838
CTCAACC9853.8943428E-48.0926731
CTTCAGT13151.7489914E-57.71503351
CTTAAGA10450.00617143566.9345651
CTCCGAG10700.0077577816.7725431
GTTAAAG16705.7030808E-56.50894071
GTTAAAT19057.676086E-66.46681
CTCAAAT19551.1468777E-56.30140921
CTAATAT15650.00121215646.0195571
CCCTGAA19253.9908165E-45.6467171
CTTTAAG17000.00325048655.54153351
CTTCAAT30454.336489E-75.4736381
CTCCGAA17250.00386266045.46122171
GTTTTAA27902.8135637E-65.4544461
CTAAAAT24154.921646E-55.40120841
CCTGAAT26654.2065687E-55.16644671
CTACAAT19700.00324661545.1498831
GTACAAT18500.0087732725.092221
CCCATAT18550.0090533735.07849451
CTTAAAT22907.376191E-45.06314131
Produced by FastQC (version 0.11.5)