FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057140
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAT8707.04933E-710.92598151
CACACAT5800.002752185310.0855221
CCCGGTT7251.5073491E-410.0855211
CCCTGTT7550.00243788528.7162951
ATTTGGC9201.940774E-48.630936
CTCATCT10901.1858606E-48.04991151
CTCCGCT8350.0060480077.88120031
CTTAATT10255.4147857E-47.8470271
CTTCTAA9500.00222831067.69684551
CTTCAAG13401.989705E-57.6394061
GTCTTGC9450.00240048677.63872586
CTTAAAG10350.00514683837.0647371
CTAAAAT13752.2556858E-46.9131661
CTTTAAT19056.656617E-66.52514651
CTCAAAA14755.3115917E-46.4444771
CTTCTAC12500.0044225456.4345621
CACATTT12800.00647205256.20348073
TACAACA15650.00126776065.9962787
CATCAAT22351.2311402E-55.88886171
CCTGCAT17150.00323759465.54262641
Produced by FastQC (version 0.11.5)