FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724370
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACT3200.007333319613.5588711
CCCAAAA7201.293383E-511.047971
CCCAAAT4800.008493341510.5457891
CTCAAGG5650.002426202610.2391481
CCCAGAT6600.0087394788.7653311
CAACAAC9853.9736752E-48.0756731
CTTCAAC10457.4884715E-47.6119981
CTATAAA10050.00430250177.19542071
CCAGATA10400.0060978936.94174672
CTATTAT11550.00216571646.8870461
CTTCCGG14758.165323E-56.86371
CTTTAAT10900.0094470656.63431071
CTTTTAA12750.00609452656.23885351
CTTTTAT15600.00119261926.0261651
CTCTTTT17800.00571164775.28135821
Produced by FastQC (version 0.11.5)