FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937782
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT9502.1193773E-710.68304351
ACCACTA7051.284149E-410.2477698
CTCCATT19200.010.19421
CTTTAAT20750.010.1314231
CCCAAGA11051.5864134E-79.8405541
CCCCATT11252.0598964E-79.665611
CTCCAAT16309.276846E-119.3394711
CCCACGG16309.2040864E-108.8947341
CTTCAAT26900.08.8930811
CTTAATT10851.3196393E-58.6856871
CCCACTT10155.715558E-58.5704931
CTCGAAT9402.3423851E-48.4831161
CCTCTCT12055.405447E-68.4223171
CTTATTT25050.08.3922971
CTCCGCT19402.8703653E-97.84708171
CCACTAC9400.00228622467.67749749
CTTAGAA8500.007669617.6756321
CTCATGT9450.00230493937.67112061
CTTAAAC8700.0094404347.4991811
CTTCTAA10709.4070134E-47.4524571
Produced by FastQC (version 0.11.5)