FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1751662
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC6350.0064602469.0956495
CTCAGGA9502.4080058E-48.4613421
CCCATAT12709.821706E-68.0555361
CCCAAAT11954.0088984E-57.94960551
CCCTCAT11251.7254986E-47.794691
CCCCATT11953.4627062E-47.33809761
CTCAAGG11450.00177260597.0203271
CTCATTT17702.1337346E-67.0185241
CCCGCGA10950.00894002156.6735361
GTCAATC13250.00129161886.53819662
CTCAAAA23255.564543E-76.2860411
CTCTATT13950.00201274386.2860411
CTCCGAT13000.00670071646.1832881
CTTTAAG13050.00697292066.15959741
CTTCTAT20251.7332624E-56.134711
CTCAAAT15509.7583025E-46.12888961
CTTTCAC19104.8749644E-56.12148481
CTCACCG16753.7293797E-46.107781
CTCGAAA14450.00298748776.06853061
GTTTGAT17004.511876E-46.0179591
Produced by FastQC (version 0.11.5)