FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945642
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT8600.0011554799.46164145
CTCAAAG21400.08.850291
GTAGCGG12906.548654E-47.709485145
GTTAAAT9600.00256495437.5879891
CTTCATT25101.8189894E-117.5456661
CCCAAAT9800.00313874647.4331321
CTTCAAT24558.367351E-117.4179931
CTTCAAA29009.094947E-127.03328131
CTCCAAT12506.0136337E-46.99309061
CTCAATC13652.1634452E-46.93758961
TTCAATT22901.0482836E-86.93055442
CCCCGTT12656.8906E-46.91016861
CTCATAC12707.207533E-46.8829631
CTCACCT10600.00673521786.8721411
CTTCAGT10600.00673521786.8721411
GTCACAT10800.0080692576.7448791
CTTAAAG14053.0810662E-46.7400791
CTTCCCT14053.0810662E-46.7400791
CCCAAGG13300.00121762756.57245351
TTAAAGC14304.2990805E-46.55842078
Produced by FastQC (version 0.11.5)