FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005223
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATG45650.0090808457.5307064145
CTCGGAT18152.8317785E-56.35426041
CTCCCAA15750.00139557015.9495551
CCCAAAT21052.4151309E-45.4788521
CAACGTT17300.00425852975.41650251
CAACGAT36952.8883733E-85.26709371
CTCGATT19300.00268708365.22868441
GTCACAT23552.1120843E-45.20330951
CTTCAAT66500.05.09445671
CCTCGAT32551.8146893E-65.09329181