FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533017
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT7054.6982677E-813.4572541
GTTCTAT13351.9445402E-57.65330461
CCTCAAT12406.455768E-57.65110061
CCCATCC8800.0098442327.46383521
CTTACAT10650.0069218266.85255671
CTCCAAT13550.00147127916.4631491
CTAAAAT16950.00288510865.59726571
GTCAGAA16000.0093793125.47347931
TTGAGGA18200.00762602175.15350069
Produced by FastQC (version 0.11.5)