FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405965
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTG6800.00105749989.5541883
CCCCCGA8659.646559E-48.3793291
CCCACGA13001.4971149E-57.8056671
CCCAAAT14004.792275E-67.7658421
CTCCAAT18105.5737473E-87.6085231
CTCAAAA29700.07.32133251
CCCAATT9900.0036403987.32133251
CTCCTGA25051.4733814E-107.23365261
CTTTAAT26758.731149E-117.0449011
CTCAGAT12707.632492E-46.84861661
CTTAAAC10650.00739996936.8057461
CTCAAAT18253.9216557E-66.75167271
CTTATAT20404.5167144E-76.75069951
CCCAGAC15501.5198682E-46.54668861
CTCAAAG29659.658834E-106.3558551
CTTTTGT34551.364242E-106.08380461
CTTCTAT24151.2734145E-66.0025831
CCCATAA15850.00140815435.94482951
CTCAAGA23354.580579E-65.8978271
CATTAAT17256.0437294E-45.88253161
Produced by FastQC (version 0.11.5)